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They tested the bacteria\’s response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier. Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm.
The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form. They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died.
This shows that antibiotic-resistant bacteria lose their resistance to antibiotics when they undergo a phase transition, right before going into the biofilm, where they will become much more resistant.
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